Figure 4. Differential expression of 10 sRNAs from the circular chromosome. Schematic diagrams of the genomic context of each sRNA are given on the left. Northern hybridizations were performed with RNA from A. tumefaciens from various growth conditions, i.e., different media, growth-phases, temperatures and pH-value, as indicated. Eight µg of total RNA were separated on a 10% polyacrylamide gel containing 7 M urea and detected by RNA gel blot analysis using RNA probes. Sizes of the sRNAs are given on the right. Ethidiumbromide-stained tRNAs were used as loading controls.