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. 2012 Jun 27;7(6):e39758. doi: 10.1371/journal.pone.0039758

Figure 4. dKDM4A-mediated H3K36me3 demethylation requires HP1a binding at a subset of heterochromatic genes.

Figure 4

(A) The expression level of endogenous dKDM4A and FLAG-tagged dKDM4A or dKDM4A-V423A. Nuclear extracts from embryos of yw, dkdm4a mutants and mutants rescued by FLAG-dKDM4A were analyzed by western blot. The doublet band is likely to represent two isoforms of dKDM4A. Asterisk indicates non-specific signal. Quantitation of the signal intensity is shown below the blot. (B) The enrichment of H3K36me3 at HP1a-bound heterochromatic genes was observed by ChIP-qPCR. Genes with lower H3K36me3 levels (Scp1, CG17374 and CG40006) are also shown in a separate panel. The primer set amplifying an intergenic region at chromosome 2 L was used as a negative control. The error bars represent standard deviation from 3 biological repeats.