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. 2012 Jun 27;7(6):e39959. doi: 10.1371/journal.pone.0039959

Figure 7. Immunocytochemical identification of cultured astrocytes dissociated from the CA1 region of the hippocampus.

Figure 7

(A) The distinct morphology of astrocytes isolated from sham-operated rats or from the ischemic hippocampal CA1 region: flat astrocytes (top) and non-flat astrocytes (bottom) cultured for 4–5 days. Both types of astroglial cells expressed GFAP and GLAST and were also positive for TRPV4. (B) The incidence of flat and non-flat astrocytes in controls (CTRL) and those isolated from the CA1 hippocampal region 1H and 7D after H/I (n =  number of coverslips). The values are presented as mean ± S.E.M. Statistical significance was calculated using one-way ANOVA. *p<0.05, significant; ***p<0.001, extremely significant.