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. 2012 Jun 27;7(6):e40152. doi: 10.1371/journal.pone.0040152

Figure 2. Fagonia cretica extract induced cell cycle arrest and apoptosis in human breast cancer cells.

Figure 2

MCF-7 and MDA-MB-231 cells were treated with 2mg/ml extract for up to 24 hours prior to cell cycle analysis using cyclin A/propidium iodide staining. (A) G0/G1 MCF-7, (B) G2 MCF-7, (C) G0/G1 MDA-MB-231, (D) G2 MDA-MB-231. (E) MCF-7 cells were treated with 2mg/ml extract for up to 72 hours prior to detection of apoptosis as annexin V positive/propidium iodide negative stained cells (Q4). Data denoted * (p<0.05), ** (p<0.01) and *** (p<0.001) are significant compared to controls (time  = 0) analysed by one-way ANOVA with Dunnett's multiple comparison post test (n = 3 independent experiments). Blots are representative of at least three independent experiments.