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. 2012 Jun 27;7(6):e40152. doi: 10.1371/journal.pone.0040152

Figure 5. Fagonia cretica extract-induced cytotoxicity is dependent on FOXO3a expression.

Figure 5

(A) MCF-7 and MDA-MB-231 cells were treated with 2mg/ml extract for up to 24 hours prior to FOXO3a protein expression analysis by SDS-PAGE and western blot. β-actin was used as a loading control. (C) MCF-7 and (D) MDA-MB-231 cells were treated with up to 2mg/ml extract for 24 hours with and without FOXO3 siRNA transfection (B). Cell viability was determined by MTT assay. Data denoted * (p<0.05), ** (p<0.01) and *** (p<0.001) are significant compared to untreated control as analysed by one-way ANOVA with Dunnett's multiple comparison post test. Data is representative of three independent experiments.