Skip to main content
. 2012 Jun 27;7(6):e40167. doi: 10.1371/journal.pone.0040167

Figure 2. Activity of endogenous promoters compared to p7.5 and SSP in vitro.

Figure 2

BHK cells were infected with 1 pfu/cell of recombinant MVA carrying tPA-Pb9-rLuc8PV under the control of the indicated promoters. Renilla luciferase activity in the culture supernatant was quantified at 8 h post-infection. Cells were either untreated (grey bars) or exposed to 40 µM AraC during and after infection (open bars), to inhibit post-replicative gene expression. Data shown are the mean and standard deviation of duplicates after subtraction of signal at 1 h post-infection and are representative of two independent experiments.