Figure 5.

(A) Timecourse of activity of mH5 promoter compared to p7.5 in vitro. BHK cells were “spinoculated” (see Materials and Methods) with 1 pfu/cell of recombinant MVA carrying tPA-Pb9-rLuc8PV under the control of the indicated promoters. Renilla luciferase activity was quantified in the inoculum and then in the culture supernatant at various time points post-infection. Cells were either untreated or exposed to 40 µM AraC during and after infection as indicated, to inhibit post-replicative gene expression. Data shown are the mean and standard deviation of duplicates. The data shown are representative of two independent experiments. (B) Cellular immunogenicity of recombinant MVA antigen (tPA-Pb9-rLuc8PV) driven by mH5 compared to p7.5 in a single-shot vaccination regimens. BALB/c mice were immunized i.m. with 10⁶ pfu rMVA and splenic CD8⁺ T cell responses to Pb9 peptide were determined 7 days later by intracellular cytokine staining and flow cytometry. Circles represent the responses of individual mice, with lines indicating the mean and the error bars showing SEM. See text for statistical analysis. The data shown are representative of two independent experiments.