Figure 1. Manufacture of esiRNA by means of a magnetic bead-integrated chip.

A. The schematic diagram shows the chip composed of a microwell array and magnetic beads coated with streptavidin. B. Large-scale manufacture of esiRNAs can be divided into three steps: target amplification and immobilization, transcription, and enzymatic digestion. C. Confirmation of PCR reactions using biotinylated or non-biotinylated primers. Amplification products (left), transcription products (middle), or esiRNA products (right) are detected only when biotinylated DNA primers are used. B: biotinylated primers.