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. 2012 Jun 27;7(6):e38085. doi: 10.1371/journal.pone.0038085

Figure 2. Bat3 knockdown reduces the extent of p21 phosphorylation following nocodazole treatment.

Figure 2

(A) Western blot for p21 using lysates from scrambled control (SC) and Bat3-knockdown (Bat3-KD) U2OS stable cell lines treated with 100 ng/ml nocodazole. The slower migrating band corresponds to the phosphorylated form of p21. (B) Western blot for p21 for parental U2OS and HCT116 cells treated with nocodazole as previously described [23]. (C) Western blot for p21 for parental U2OS cells treated with 800 ng/ml nocodazole for 24 h. siRNA was used to transiently reduce Bat3 expression (right panel). Lysates from the stable, inducible U2OS cell lines were used as a positive control (left panel).