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. 2012 Jun 27;7(6):e38085. doi: 10.1371/journal.pone.0038085

Figure 4. Hyperphosphorylation and abundance of p21 protein in the U2OS stable cell lines upon release from nocodazole arrest into CHX and MG132.

Figure 4

(A) Western blot for p21 for lysates from scrambled control (SC) and Bat3-knockdown (Bat3-KD) U2OS cells arrested with 100 ng/ml nocodazole for 24 h and subsequently released into full culture medium containing 40 µg/ml CHX. (B) Western blot for p21 for lysates from SC and Bat3-KD U2OS cells arrested with 100 ng/ml nocodazole for 24 h and subsequently released into full culture media either with or without 20 µM MG132. For the MG132 samples, cells were pre-treated with 20 µM MG132 for 2 h before release. A, asynchronous population; CHX, cycloheximide.