Figure 5. Bat3 knockdown causes a defect in the oscillation of p21 protein level in G1/S-synchronized cells.

(A) Western blot for p21 for scrambled control (SC) and Bat3-knockdown (Bat3-KD) U2OS cells following release from G1/S synchronization. (B) Western blot for p21 for G1/S-synchronized SC and Bat3-KD U2OS cells that were released into full culture media either with or without 20 µM MG132. For the MG132 samples, cells were pre-treated with 20 µM MG132 for 2 h before release. (C) Western blot for p21 for G1/S-synchronized SC and Bat3-KD U2OS cells that were released into full culture medium containing 40 µg/ml CHX. A, asynchronous population; CHX, cycloheximide.