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. 2012 Jun 27;7(6):e39514. doi: 10.1371/journal.pone.0039514

Figure 5. Functional activity of NK cells derived from co-culture with MS-5/SP-HOXB4.

Figure 5

(A) The functional activity of cells was assessed in the presence of IL-12 and against K562 target cells; the NK-cell activity was evaluated by the mobilisation of the CD107a antigen on the cell surface and the intra-cytoplasmic expression of IFNγ. Cells were gated on CD56+ cells (not shown). (B) Cytotoxic activity of NK effector cells against target K562 cells. a) Target K562 cells were labelled by CFSE. b) Percentage of 7AAD+ death cells among the CFSE+ target cells at various Effector-Target (E/T) ratios (one experiment out of two). c) Percentage of cytotoxicity (i.e percentage of 7AAD+ death cells among the CFSE+ target cells) according to the ratio E/T (dotted line and bold line represent two independent experiments).