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. Author manuscript; available in PMC: 2013 Apr 27.
Published in final edited form as: J Nat Prod. 2012 Apr 6;75(4):834–851. doi: 10.1021/np200993k

Figure 1. Graphical representation of the qHNMR concept and work-flow.

Figure 1

Using 1H signals for universal detection, qHNMR is amenable to a broad range of pure to crude natural products (NPs). The qHNMR method consists of three main components: (i) ACQU – the acquisition, comprised of a suitable 1H NMR experiment (expt) that establishes quantitative conditions (qCond); (ii) CAL - quantitative calibration using a suitable external calibrant and method, or an internal calibrant; (iii) PROC – post-acquisition processing of the raw NMR data (i.e., FIDs), using a standard operation protocol (SOP) and software tool (soft). Major strengths of the qHNMR work-flow include the ability to simultaneously yield concentration (conc) and identity (ID) information, and to perform the analysis for several target analytes at a time and without the need for identical calibration standards.