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Total Kiss1 mRNA in the AVPV/PeN of OVX mice with (a) and without (b) E2 treatment that were sacrificed at different circadian times. In the OVX+E2 mice, an LH surge was detected at CT 11 and CT 12 (see Robertson et al., 2009). (*) indicates a statistical significance of p<0.05 relative to CT 0, CT 4, CT 8, and CT 20.
