Generation and characterization of UBC-HS-GR transgenic mouse embryos. A, Schematic of the UBC-HS-GR gene construct. cHS4 = chicken hypersensitive site 4; SS = endoplasmic reticulum signal peptide; pA = bovine growth hormone polyadenylation signal. B, In each panel, UBC-HS-GRtg/+ (left) and wild type (right) E11.5 embryos viewed under a stereofluorescence microscope. C, Confocal images of embryonic tissues from UBC-HS-GRtg/+ mice. Left panels, H2B-EGFP; center panels, mCherry-GPI; right panels, composite images. Arrows indicate mitotic cells. Scale bar = 30 μm.