Table 1.

List of phosphopeptides featuring the unusual fragment ion (From refs. 8-10)

Gene	Protein	Peptide Sequence	aBP %	bx/y-98
PS(Phospho)-containing sequences
A3KGU4_MOUSE	Spectrin alpha 2	TSSKE(ss)PVPsPTLDRc	50	4.4
A3KGU4_MOUSE	Spectrin alpha 2	E(ss)PVPsPTLDR	28	3.5
A3KGU4_MOUSE	Spectrin alpha 2	RPPsPDPNTK	5	6.7
SPTB2_MOUSE	Spectrin beta chain, brain 1	(tssKEss)PVPsPTLDR	80	4
ANK2_MOUSE	Ankyrin-2	TEWQPsPTDIPLQK	20	4.7
B2RQQ5_MOUSE	Microtubule-associated protein 1B	SPSLSPSPPsPIEK	30	0.3
MAP1A_MOUSE	Microtubule-associated protein 1A	AELEEMEEVHPsDEEEEETK	9	1.8
NFH_MOUSE	Neurofilament heavy polypeptide	IGFGPsPFSLTEGLPK	14	1.8
B1ART6_MOUSE	Microtubule-actin crosslinking factor 1	LLDAEDVDVPsPDEK	BP	8.9
SYGP1_RAT	Ras GTPase-activating protein SynGAP	LPsPTK	BP	6.4
LRRC7_MOUSE	Leucine-rich repeat-containing protein 7	TPsPFEDR	43	2
KPCG_MOUSE	Protein kinase C gamma	MGP(sss)PIPSPSPsPTDSKR	(2+)BP (+) 69	3.3 3
LMNA_MOUSE	Prelamin-A/C	LSPsPTSQR	BP	4.6
STE20_CANAL	Serine/threonine-protein kinase CST20	IPsNPTSTR	52	1.8
STE20_CANAL	Serine/threonine-protein kinase CST20	TPsSQSLPR	18	0.4
PMA1_CANAL	Plasma membrane ATPase 1	GYLVAMTGDGVNDAPsLK	29	7.3
ACT_CANAL	Actin	EITALAPsSMK	18	2.5
TSA1_CANAL	Peroxiredoxin TSA1	YGEVCPANWHPGDETIKPsPEASK	7	y-98 not detected
TSA1_CANAL	Peroxiredoxin TSA1	APVVQQPAPsFKK	16	5
RS13_CANMA	40S ribosomal protein S13	NAPsWFK	9	3
RAD18_CANAL	Postreplication repair E3 ubiquitin- protein ligase RAD18	ANVASPsPVAQSTVHK	12	y-98 not detected
PT(Phospho)-containing sequences
B2RQQ5_MOUSE	Microtubule-associated protein 1B	DLTGQVPtPPVK	52	2.8
RBM14_MOUSE	RNA-binding protein 14	QPtPPFFGR	5	0.3
TBA1B_MOUSE	Tubulin alpha-1B chain	sIQFVDWCPtGFKd	13	2.8
LRFN6_MOUSE	Leucine-rich repeat and fibronectin type- III domain-containing protein 6	VFSLDVPDHPtPTGLAKd	16	1.5
SSN2_CANAL	Mediator of RNA polymerase II transcription subunit 13	SNDYAPtPMIQDK	5	y-98 not detected
CHS5_CANAL	Chitin biosynthesis protein CHS5	IESVPtDEIDTK	4	3
STE7_CANAL	Serine/threonine-protein kinase STE7 homolog	ILHIPtQK	23	1

^athe relative intensity of the ‘x’ fragment within the spectrum; BP = base peak

^bthe intensity ratio of the ‘x’ fragment and the normal y-H₃PO₄ fragment

^camino acids in lower case indicate phosphorylation sites; multiple residues in parentheses indicate ambiguity of the site assignment.

^diTRAQ-labeled peptides