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. Author manuscript; available in PMC: 2013 Apr 13.
Published in final edited form as: Cell. 2012 Apr 13;149(2):348–357. doi: 10.1016/j.cell.2012.01.057

Figure 4. Free energy landscape for folding.

Figure 4

Energetic perturbations in the Azoarcus ribozyme due to mutations in IC (red) and N (blue) are shown versus Mg2+ concentration (back to front). Mutations that destabilize N more than IC, e.g. J2/3 and most of triplets, shift the crossover of surfaces to higher Mg2+ compared to WT. Red and blue surfaces are similar because IC is similar in structure to N. Single mutations located in helical junctions (J2/3 and TH) are particularly destabilizing. See also Figure S4.