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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Immunol. 2011 Nov 23;188(1):445–453. doi: 10.4049/jimmunol.1101224

Figure 3.

Figure 3

Adenosine increases IL-10 production via A2BARs.

BV-2 microglia were treated with increasing concentrations of CCPA, CGS21680, NECA or IB-MECA and 20 μg/ml PGN (A) or 10 μg/ml LPS (B) for 24 hours, and IL-10 concentrations were determined from the supernatants obtained after the 24-hour incubation period. ***p<0.001 vs. vehicle. C. BV-2 cells were treated with NECA or its vehicle and PGN for 24 hours, and IL-10 concentrations were determined from the supernatants with ELISA. ***p<0.001 vs. vehicle, ###p<0.001 vs PGN. D. BV-2 cells were pre-treated with 0.1 μM AR antagonists DPCPX (A1AR), ZM241385 (A2AAR), MRS1754 (A2BAR) or MRS1523 (A3AR) 30 min prior to treatment with 10 μM NECA and 20 μg/ml PGN (D) or 10 μg/ml LPS (E). After 24 hours, supernatants were removed and IL-10 concentrations were determined from the supernatants using ELISA. Results are expressed as percent of vehicle (group treated with NECA and PGN). ***p<0.001 vs. vehicle.