PGN or LPS treatment augments A2BAR mRNA expression
BV-2 cells (A) or primary microglia (B) were treated with 20 μg/ml PGN or 10 μg/ml LPS for 4 hours, after which period RNA was isolated from the cells. RNA was then reverse transcribed to cDNA. Real-time PCR was performed using the cDNA samples and primers specific for A1AR, A2AAR, A2BAR, A3AR or 18S. Results were normalized to 18S mRNA content. ***p<0.001 vs. vehicle, &&p<0.01 vs. vehicle, ###p<0.001 vs. vehicle, $$p<0.01 vs. vehicle. Results (mean ± SEM) shown are representative of three separate experiments (n=4 in each experiment).