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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biometals. 2012 Mar 21;25(4):665–675. doi: 10.1007/s10534-012-9538-6

Fig. 3. Reduction of TBI and NTBI is dependent upon hBMVEC-surface ferrireductases.

Fig. 3

Colorimetric ferrozine assays were used to quantify the formation of FeII species in solution. Each sample was blank corrected with a minus hBMVEC control and normalized for protein concentration. Potassium tetrachloroplatinateII (100 μM) was used to inhibit reductase activity by hBMVEC. Substrates were either TBI (a) or NTBI (b). Values are mean ± SD (a, n =12; b, n=3). ***P-value < 0.0001 as analyzed by the paired t-test.