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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biometals. 2012 Mar 21;25(4):665–675. doi: 10.1007/s10534-012-9538-6

Fig. 4. Both TBI and NTBI are substrate for endosomal-independent hBMVEC iron uptake.

Fig. 4

A γ counter was used to quantify the amount of 59Fe uptake by hBMVEC monolayers. The kinetics of 59FeII-citrate plus Asc accumulation by hBMVEC are shown (a). Data are shown representing hBMVEC accumulation of 59Fe from 59Fe-Tf in the presence ferrozine (b) or PtII (c). 59NTBI uptake in the presence of PtII was also monitored (d). Effect of hBMVEC-alkalinization via NH4Cl on 59TBI accumulation was quantified and compared to control (e). n = 3 for each result in each panel. The Michaelis-Menten equation was used to calculate the curve (a). Data are mean ± SD. *P-value < 0.01 as analyzed by the unpaired t-test.