Table 1.
Evaluation of Nanozyme Entrance, Intracellular Trafficking, and Release by % Colocalization with Specific Intracellular Compartments in Carrier Cells, Human Macrophages
| Organelle | 5 min | 15 min | 30 min | 60 min |
|---|---|---|---|---|
| Entrance | ||||
| clatrin-coated pits | 35.1 ± 2.2b | 29.0 ± 1.1 | 21.6 ± 3.4 | 43.0 ± 3.9 |
| caveolae | 4.9 ± 1.9 | 2.2 ± 1.2 | 2.9 ± 0.2 | 1.3 ± 0.2 |
| Intracellular trafficking | ||||
| Acidic organelles | 49.5 ± 6.2 | 56.6 ± 4.1 | 58.4 ± 3.4 | 61.0 ± 3.9 |
| ER | 18.3 ± 5.0 | 10.2 ± 4.9 | 14.6 ± 1.4 | 16.8 ± 1.5 |
| Mitochondria | 12.5 ± 1.6 | 9.7 ± 1.0 | 18.5 ± 2.3 | 17.0 ± 2.0 |
| Early endosomes | 13.0 ± 4.9 | 4.68 ± 2.2 | 7.2 ± 1.4 | 9.0 ± 0.8 |
| Late endosomes | 13.4 ± 1.5 | 9.9 ± 1.7 | 30.0 ± 3.8 | 24.7 ± 3.2 |
| Recycling endosomes | 35.0 ± 1.5 | 26.1 ± 2.2 | 26.2 ± 2.1 | 47.4 ± 9.5 |
| Lysosomes | 23.4 ± 6.7 | 11.13 ± 1.9 | 19.6 ± 3.8 | 35.8 ± 4.9 |
| Release a | ||||
| non-activated | activated with LPS and IFN-γ | |||
| Actin microfilaments | 26.1 ± 0.1 | 79.1 ± 7.2 | ||
a
nanozyme-preloaded murine macrophages were incubated with CATH.A neurons for 18 hours
b
Data represent means ± SEM (N = 40).