. Author manuscript; available in PMC: 2013 Jun 1.

Published in final edited form as: Nanomedicine (Lond). 2012 Jan 11;7(6):815–833. doi: 10.2217/nnm.11.156

Table 2.

Evaluation of Nanozyme Entrance and Intracellular Trafficking by % Colocalization with Specific Intracellular Compartments in in Target Cells, HBMEC

Organelle	from macrophages^a		free, from media
	30 min	60 min	30 min	60 min
*Entrance*
actin	13.1 ± 2.5 ^b	19.0 ± 1.4	n/a	n/a
tubulin	58.0 ± 4.3	44.5 ± 2.5	n/a	n/a

*Intracellular trafficking*
ER	9.6 ± 21.7	16.3 ± 1.0	18.7 ± 1.9	19.1 ± 1.0
Mitochondria	8.9 ± 1.1	6.9 ± 0.7	1.1 ± 0.3	0.7 ± 1.4
Early endosomes	4.2 ± 0.9 (^*)	17.2 ± 1.6 (^*)	15.2 ± 3.3	26.9 ± 1.7
Late endosomes	29.5 ± 1.4 (^**)	22.4 ± 1.3 (^**)	4.3 ± 0.7	12.2 ± 2.1
Recycling endosomes	9.5 ± 1.4 (^*)	11.8 ± 1.1 (^*)	4.4 ± 0.4	19.7 ± 2.1
Lysosomes	9.6 ± 2.4 (^*)	11.0 ± 2.0 (^*)	27.8 ± 4.4	19.4 ± 2.4

Statistical significance compared to colocalization levels of macrophages-free nanozyme accumulated from the media is shown by asterisk: p<0.05 (*); p<0.005 (**).

Data represent means ± SEM (N = 40).