Table 1.
microRNA differentially expressed in control vs. T- or T plus flutamide-treated fetal ovine ovaries
| miRNA | C vs. T FC | C vs. TF FC | Functional analysis literature based$ | Predicted targets (Targetscan) |
|---|---|---|---|---|
| miR-497 | 3.83 | 3.71 | Type 2 diabetes rat [miR-15b/497 family (1) | PAPPA, IR, GHR, IGF2R, IRS2, IGF1R, furin |
| miR-29a | 2.98 | Diabetes/insulin signaling (1–5), FSH regulated (6) | IGF1, INSIG1, leptin | |
| miR-192 | 2.19 | Diabetic neuropathy (4, 7, 8) | IGF1 | |
| miR-24-2* | 2.12 | Diabetes (9), Insulin signaling (10), bovine ovary (11) | INSIG1, PPARa, Furin, IGFBP5, IGF2BP2 | |
| miR-15b | 2.06 | 2.93 | Type 2 diabetes rat FSH (1), regulated in ovary (6) | PAPPA, IR, GHR, IGF2R, IRS2, IGF1R, furin |
| miR-101 | 1.90 | AR regulated (12) | PGRMC2, PPARa | |
| miR-212 | 1.87 | LH regulated in ovary (13) | ||
| miR-451 | 1.85 | Sex dependent in liver (14) | ||
| miR-186 | 1.82 | Type 1 diabetes (15) | INSM1, LEPR, IGF1, IGF1R | |
| miR-672 | 1.82 | Mouse ovary (16) | IGF1R | |
| miR-7 | 1.67 | Insulin signaling (17, 18) | IRS2, IRS1, IGF1R, PAPPA | |
| miR-30b-5p | 1.58 | Estrogen regulated (19) | LEPR, IGF2R, INSIG2, IRS1, IRS2, LDLR, IGF1R, IGF1 | |
| miR-22* | 1.52 | Fetal ovine gonad (20), androgen regulated (21), represses ESR1 (22) | ESR1, GHRHR, PTGS1, IGF2BP1, furin | |
| miR-378 | −4.13 | Lipid/fatty acid metabolism (23), regulates estrogen production (24) | ||
| miR-760 | −2.80 | Estrogen regulated (25) | IR | |
| miR-10a | −2.76 | −3.36 | Androgen regulated (26), ovary (27) | PPARa |
| miR-182 | −2.71 | Insulin signaling (10), ovary (27) | INSIG1, IGF2BP1 | |
| miR-129* | −2.22 | Diabetes (28) | IGF1, GHR, ESR1, INSIG2 | |
| miR-132 | −1.82 | LH regulated in ovary (13) | ||
| miR-223 | −1.69 | Diabetes (9) | IGF1R | |
| miR-363 | 4.50 | Sex differentiation (29) | PTGER4, IRS2, INSIG1 | |
| miR-20b | 3.24 | Diabetes (9), ESR1 regulated (30) | PPARa, LDLR, IGF2BP1, ADIPOR2, PPARd | |
| miR-330 | 1.71 | Fetal programming (31) | IGF2BP1 | |
| miR-29c | 1.64 | Diabetic neuropathy (32), diabetes (2) | IGF1, INSIG1, leptin | |
| miR-29b | 1.62 | Diabetes (2, 9), sex dependent in liver (14) | IGF1, INSIG1, leptin | |
| miR-191* | 1.56 | Diabetes (9) | ||
| miR-101a* | 1.53 | AR regulated (12) | PGRMC2, PPARa | |
| miR-105 | 1.52 | Human ovary (27) | ||
| miR-133a | −1.85 | Insulin signaling (33, 34) | IR, IGF1R |
C, Control; ER, estrogen receptor; FC, Fold change; TF, testosterone + flutamide.
References for table are included in the Supplemental Online Materials.