Figure 1.

Effects of CAM on RSV G mRNA expression (a) and production of infectious virus particles (b) in A549 cells infected with RSV. One hour before RSV infection, CAM was added to A549 cell culture at the indicated concentration. A549 cells were infected with the RSV at MOI of 1. (a) RT-PCR. After 24 h of infection, total RNAs were extracted from the cells. The mRNA levels of RSV G were determined by RT-PCR. The mRNA levels of human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were carried out as a control. (b) Plaque-forming assay. After 24 h and 36 h infection, the culture supernatants were corrected. Virus titers in the supernatants were determined by plaque-forming assay using Hep-2 cells as the indicator cell. Each experiment was performed in quadruplicate. The mean value and standard deviation are shown.