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. 2012 Jun 13;8(6):859–869. doi: 10.7150/ijbs.3490

Figure 3.

Figure 3

Expression of human CIP4 (hCIP4) is upregulated in the HK-2 cells treated with TGF-β1. (A) Representative RT-PCR analysis for hCIP4 mRNA expression in the HK-2 cells treated by TGF-β1 for 0 h, 12 h, 24 h, 48 h, and 72 h. The expression of hCIP4 mRNA was increased in TGF-β1-treated cells. The histogram shows the average relative amounts of mRNA density corrected for the loading control, GAPDH (n = 4).*P<0.05 compared with the HK-2 cells treated by TGF-β1 for 0 h. (B) Representative Western blot analysis of hCIP4 in the HK-2 cells treated by TGF-β1 for 0 h, 12 h, 24 h, 48 h, and 72 h. The expression of the hCIP4 protein was increased in TGF-β1-treated cells. The histogram shows the average volume density corrected for the loading control, GAPDH (n = 4).*P<0.05 compared with the HK-2 cells treated by TGF-β1 for 0 h. (C) Representative immunofluorescence of hCIP4 localization in a monolayer of control cells and TGF-β1-treated cells. Arrows indicate positions of CIP4 in HK-2 cells. Cell nuclei were enhanced by staining of cell nuclei with DAPI. Scale bars represent 20μm.