Figure 1.

Human haematolymphoid cells in organs of engrafted BLT-NSG mice. (a) Approximately 3 months after engraftment of BLT-NSG mice with human fetal liver and thymus tissue, engraftment was assessed in target organs using flow cytometry. Initial gating strategy to identify cells in the lymphocyte gate was based on forward and side scatter profiles. Human CD45⁺ cells were next selected using antibodies directed against mouse and human CD45. Viable hCD45⁺ cells were gated by exclusion of the viability marker LIVE DEAD AQUA. T cells were next selected for by identifying CD3⁺ cells within the lymphocyte gate. B cells were identified using the phenotypic markers CD19 and CD20 on the CD3-negative population within the lymphocyte gate. Values on the y-axis represent frequencies of (b) hCD45⁺, (c) hCD3⁺ and (d) hCD19⁺ CD20⁺ and CD19⁺ CD20⁻ cells within the lymphocyte gate in the spleen (n = 16) and all cells in the bone marrow (n = 16).