Fig. 2.

Observation of spore differentiation using the monoxenic culture. Newly developing spores (ns) and swollen hyphal bodies (sb) were distinguished by their transparency, as the cytoplasm was dark and cloudy in appearance. Spores containing cytoplasm were always connected to non-septate hyphae, whereas empty-looking spores were connected to septate hyphae. A~C, Images of runner hyphae and the swollen hyphal body developed from the hyphae. The hyphae connected to the empty-looking swollen hyphal bodies were septate. One of the two swollen mycelia was filled with cytoplasm and became a new spore, whereas the other remained as a swollen body in the image of (C). At the end of spore formation, mature spores (ms) were produced in the image of (D). Septa are represented with '*.' 'sh', 'ch,' and 'fh' which represent septate hyphae, cytoplasmic hyphae (non-septate), and finely branching hyphae, respectively (scale bars = 100 µm).