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. Author manuscript; available in PMC: 2012 Sep 9.
Published in final edited form as: Science. 2012 Jan 26;335(6073):1238–1242. doi: 10.1126/science.1214956

Fig. 3. A differential excitation/inhibition balance underlying the increased activation of immature GCs.

Fig. 3

(A) Left, Schematic diagram of the recording configuration and activated circuits. Stimulation of the mPP evokes monosynaptic excitation and disynaptic inhibition via GABAergic interneurons (IN) onto GCs. Spiking and synaptic currents were subsequently measured using loose patch followed by whole-cell recordings in individual cells. Right top, example loose patch traces depicting spikes evoked by stimulation of the mPP at threshold intensity in 4 wpi and mature GCs. Right bottom, the underlying EPSCs (black traces) and IPSCs (gray traces) were recorded in whole cell at the reversal potential of the inhibition (-55 and −60 mV, respectively) and excitation (0 mV for both). Dotted lines depict the spiking time after mPP stimulation (see S.O.M.). (B) Latency to spike measured at threshold intensity displayed similar values between 4 wpi and mature GCs. (C) Peak EPSG and IPSG in 4 wpi and mature GCs (*, p < 0.02, t-test). (D) Latency to onset for EPSCs and IPSCs show a profound delay in IPSC onset for 4 wpi neurons (*, p < 0.05; **, p < 0.01, t-test). (E) EPSG and IPSG amplitudes measured at the precise time of evoked spikes (*, p < 0.05 for EPSG and p < 0.03 for IPSG, t-test). n = 4 for all experiments.