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(A) EF24 is more potent than curcumin in reducing LPS-induced TNF-α secretion from JAWS II DCs. LPS-stimulated DCs were treated with 10 μM EF24 or curcumin and secreted amounts of TNF-α (picograms per milligram, mean ± SEM) were measured by ELISA. (B) Percent binding of EF24 (0–20 μM) and polymyxin B (0–20 μM) with LPS (0.1 IU ml−1) in an in vitro microwell-based assay. Polymyxin B served as a positive control. The results are representative of two experiments performed in triplicate.
