Fig. 6.

Inhibition of LPS-induced NF-κB by EF24. (A) NF-κB-DNA binding activity in JAWS II DCs treated with vehicle, 100 ng ml⁻¹ LPS, 1 μM EF24 and post-treatment with 1 μM EF24 was investigated by EMSA. The values were normalized with those of vehicle-treated cells shown as the dotted line. * P < 0.001 versus LPS-treated cells and # P < 0.001 versus vehicle-treated cells. (B) The effect of EF24 treatment (10 μM) on LPS-induced NF-κB activity was also confirmed by luciferase reporter assay (# P < 0.05 versus control and * P < 0.05 versus LPS treatment). The results are representative of three independent experiments performed in triplicate.