Figure 3.

Long-latency excitation comes from tufted, but not mitral cells. (A) Example of a mitral cell (black) and tufted cell (blue) response to glomerular stimulation (arrowhead). (B) Distribution of first spike latencies following glomerular stimulation in mitral (black) and tufted cells (blue). (C) Optical imaging setup. A stimulating electrode was impaled into a glomerulus and a nearby (but not connected) tufted cell was patched. The glomerulus was stimulated on every trial, while the tufted cell was made to fire on every other trial. Responses from three granule cells (D–F) to glomerular stimulation with (blue) and without (gray) additional tufted cell input were monitored optically. Black dash denotes timing of glomerular stimulation, blue dash denotes onset of tufted cell activity for tufted stimulation trials (blue).