Fig. 4.

Agonist-stimulated IP production by mutants of the hGnRHR after pharmacoperone rescue. Functional activity was assessed for mutants with K¹⁹¹ (the WT condition) (A) or without K¹⁹¹ (B). Cos-7 cells were transfected with 10 ng of WT or mutant cDNA, with or without K¹⁹¹ as described in Materials and Methods. After rescue, IP production was measured in response to 10⁻⁷ m buserelin (metabolically stable GnRH agonist). In all figures, sem are shown for least three independent experiments performed in replicates of four to six.