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FST ND-FSD1-FSD1 is a unique, specific myostatin antagonist as shown through transient expression in luciferase reporter assays. Increasing amounts of FST-type DNA were transfected into HEK293-CAGA cells, which were later treated with exogenous ligands. Total DNA was normalized using empty vector. Each condition was performed in triplicate for each experiment, and each experiment was performed twice. Figures represent the combined data from all experiments. Error bars represent ±sem.
