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The double point mutation F47D/F52D negatively affects inhibition by FST, especially in the absence of FSD3. Purified proteins expressed from the corresponding FST-type constructs were mixed with ligands and applied to HEK293-CAGA cells. Each condition was performed in triplicate for each experiment, and each experiment was performed three to four times. Figures represent the combined data from all experiments. Error bars represent ±sem.
