Fig. 1.

Effect of dexamethasone (DEX), mifepristone (RU486), cycloheximide (CHX), actinomycin D (ActD) or selected combinations on promoter specific transcription of (HUMAN)NAT1 gene. Primary human hepatocytes were kept in DEX-free medium for 24 h and then treated for 24 h. (HUMAN)NAT1 mRNA levels were determined by real-time RT-PCR and normalized to (HUMAN)GADPH mRNA level. Values represent the mean ± SD of results from two independent experiments of a single culture of human hepatocytes. a Effect of DEX concentration on (HUMAN)NAT1 gene expression driven by P1 (filled triangles), P2 (empty squares), or P3 (diamonds, female donor primary hepatocytes; error marks, male donor primary hepatocytes). b Effect of RU486 (10 μM) and CHX (20 μM) on P3-mediated (HUMAN)NAT1 gene expression with or without DEX (10 μM). c Effect of ActD (5 μM) on promoter 3-mediated transcription of (HUMAN)NAT1 gene with or without DEX (10 μM). *p ≤ 0.01, significantly different from cells treated with DEX; **p ≤ 0.01, significantly different from cells treated with carrier solution (control)