Fig. 3.

Identification of the putative GREs in the P3-containing region of the (HUMAN)NAT1 gene. a Effect of the length of 5′-flanking region of P3 on DEX and glucocorticoid receptor mediated induction of luciferase gene expression. A series of deletion reporter constructs were made by inserting a desirable P3-containing fragment into pGL3-basic vector. HepG2 cells were co-transfected with the new plasmid and pCMV-hGRα (solid bars) at 1 μg each per well. The controls were co-transfected with empty plasmid without hGRα (open bars). Cells were transfected for 12 h, maintained in regular medium for 24 h, and treated with DEX (1 μM) for additional 24 h before cell lysis and luciferase assay. Values represent the mean ± SD of three independent transfections. **p ≤ 0.01, significantly different from control transfected with empty vector. b Putative 3′- or 5′-half of GREs (underlined) in the region between −690 and −801 bp upstream of exon 1