Table 1.
Erythrocyte membrane proteins identified by MALDI-TOF analysis.
Spot | Protein | Mass | Accession No. | Score | Matched peptide |
---|---|---|---|---|---|
1 | α-Spectrin | 281 | P02549 | 2792 | 63 |
2 | β-Spectrin | 246 | P11277 | 2280 | 55 |
3 | Band 3 | 102 | P02730 | 301 | 17 |
4 | Protein 4.1 | 97 | P11171 | 1386 | 42 |
5 | Band 3 fragment | 102 | P02730 | 251 | 8 |
6 | P55 | 52 | Q00013 | 332 | 19 |
7 | α-Spectrin | 281 | P02549 | 158 | 4 |
8 | Flotillin 1 | 47 | O75955 | 212 | 6 |
9 | Band 3 cyt domain | 42 | P02730 | 345 | 21 |
10 | Actin-β | 41 | P60709 | 354 | 16 |
11 | Dematin (protein 4.9) | 46 | Q08495 | 299 | 7 |
12 | GAPDH | 36 | P04406 | 312 | 12 |
13 | Tropomyosin 3 | 29 | P06753 | 372 | 18 |
14–15 | Band 3 cyt domain | 42 | P02730 | 345 | 21 |
16 | Catalase | 59 | P04040 | 300 | 15 |
Spot numbering corresponds to the spot numbers in the fluorescein-5-maleimide and anti-phosphotyrosine antibody 2-DE gels in Fig. 1.