Table 2. FISH-validated genes required for normal somatic homolog pairing.

Gene Targeted	Gene Function	Validation Amplicon	% Paired Nucleia	Nuclei Scored	Nuclear Sizeb	% Nuclei > 4 Signalsc
Control	—	(water)	66.8	4498	2537 ± 892	0.002
β-Tub56D	Cytoskeleton	DRSC33236	47.9	361	2943 ± 1330	1.1
CG31635	Unknown	DRSC31568	54	274	2573 ± 787	0.36
CG5844	Fatty acid metabolism	DRSC30977	52.3	151	2417 ± 925	0
chb	Microtubule binding	DRSC36464	57.7	345	2643 ± 832	0.29
feo	Cytokinesis	DRSC23773	53.1	241	2892 ± 1598	3.7
geminin	Regulation of DNA replication	DRSC33311	56.3	190	3879 ± 1886	2.1
His3	Core histone	DRSC40904	57.6	184	2848 ± 1041	0.54
ial-1	Aurora B kinase; cytokinesis	DRSC36311	45.4	207	3360 ± 2048	6.3
Incenp	Inner centromere protein	DRSC39343	46.6	161	3637 ± 2297	9.9
Klp61F	Mitotic kinesin	DRSC30823	31.7	123	3727 ± 1967	13.9
Myb	Oncogene	DRSC31099	39.7	209	3315 ± 1843	12
pav	Kinesin; cytokinesis	DRSC33333	33.8	136	2972 ± 1903	8.8
pbl	Cytokinesis	DRSC33335	41.5	299	2776 ± 1229	3.7
polo	Kinetechore	DRSC34463	46.7	244	2628 ± 1180	2
scra	Contractile ring	DRSC07679	41.4	280	3313 ± 1807	8.2
Sse	Mitotic chromosome segregation	DRSC34476	53.6	308	2777 ± 1229	0.32
tsr	Actin binding protein	DRSC31277	55.6	144	2289 ± 1256	4.1

^aPercentage of nuclei with a single FISH signal for the 359 bp repeat.

^bNuclear sizes were determined from images representing flattened z-stacks and are presented as mean areas of DAPI staining plus or minus the standard deviation. Units are arbitrary.

^cPercentage of nuclei with greater than four FISH signals for the 359 bp repeat, which are assumed to be polyploid/aneuploid.