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Expression of the RAS2Val19 dominant allele is detrimental to all kinetochore mutants, whereas deletion of RAS2 rescues a subset of kinetochore mutants. (A–C) Cell dilution assay of the indicated strains grown on YPD plates at the indicated temperatures for 3 days. (D) Cell dilution assay of wild-type cells and the indicated mutants carrying the plasmid vectors pRS326 or pRAS2val19 grown on SC-URA plates at the indicated temperatures for 3 days.
