Figure 3.

Kinetochore mutants have higher levels of Msn2 PKA-dependent phosphorylation. Cells were grown to mid-logarithmic phase at 25°C (A) or the semipermissive temperature of 31.5°C (B) and lysed for immunoblot analysis. The blots were probed with α-P-CREB antibody to detect PKA-dependent Msn2 phosphorylation (P-Msn2) and α-Msn2 and α-Pgk1 as loading controls. One representative blot is shown out of three independent experiments. The bar graphs are the relative ratio of P-Msn2/total Msn2 analyzed from the three independent experiments. The value of 1 was assigned to the ratio of P-Msn2/total Msn2 in the wild-type cells. The asterisk represents values that are significantly different from the wild-type value (P < 0.05).