Figure 4.

PKA substrates have higher levels of phosphorylation in kinetochore mutants. (A) The lysates described in Figure 3 were probed with the anti-PKA substrate antibody (α-sub) and α-Pgk1 as a loading control. Two protein bands from the α-sub blot, Pa and Pb, were selected for quantification. One representative blot from three independent experiments is shown. Bar graphs are the relative ratio of Pa/Pgk1 or Pb/Pgk1 averaged from three independent experiments. The value of 1 was assigned to the ratio of Pa/Pgk1 or Pb/Pgk1 in the wild-type cells. The asterisk represents values that are significantly different from the wild-type value (P < 0.05).