Table 1. Reduction of PKA activity rescues CF loss in the ndc10-1 kinetochore mutant.
Genotype (MATa) | Total Colonies | Chromosome Loss(Red) | Chromosome Loss in First Division (Red/White Half Sectors) |
---|---|---|---|
ndc10ΔHIS3 ndc10-1::kanMX6 CFIII | 11,632 | 6.9 × 10−2 (801)a | 1.3 × 10−2 (143) |
ndc10ΔHIS3 ndc10-1::kanMX6 ras2ΔLEU2 CFIII | 11,118 | 4.1 × 10−3 (46) | 6.3 × 10−4 (7) |
ndc10ΔHIS3 ndc10-1::kanMX6 CFIII pRS416 | 5,926 | 1.8 × 10−2 (104) | 8.3 × 10−3 (49) |
ndc10ΔHIS3 ndc10-1::kanMX6 CFIII pRS416-BCY1 | 6,409 | 9.7 × 10−3 (62) | 1.9 × 10−3 (12) |
Numbers in brackets represent the total number of red or red/white colonies.