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Modified schematic map of PCV2 ORF3 DNA sequence expressed in E. coli. The mutated nucleotide is in gray highlight. A fragment of 108 bp in length was obtained with the primers F3GZU1 and F3GZD1, while a fragment of 227 bp in length was amplified with the primers F3GZU2 and F3GZD2. Modified DNA sequence of ORF3 was generated by primers F3GZU1 and F3GZD2 from fragments of 108 bp and 227 bp.
