Skip to main content
. 2012 Jun;31(3):180–187. doi: 10.1089/hyb.2011.0116

FIG. 3.

FIG. 3.

PCR, SDS-PAGE, and Western blot analyses of in vitro expression of ORF3-encoding protein. (A) PCR amplification of rebuilt ORF3. Lane 1, 315 bp fragment of the rebuilt ORF3; lane 2, 108 bp fragment; lane 3, 227 bp fragment; lane M, 100 bp marker. (B) SDS-PAGE analysis of ORF3-encoding protein expressed in E. coli. Lane P, protein marker; lane 1, cell lysates of E. coli strain BL21; lane 2, cell lysates of E. coli strain BL21 transformed with the vector pGEX-4T-1; lane 3, cell lysates of E. coli strain BL21 transformed with the recombinant plasmid pGEX-4T-1-F3; lane 4, purified GST-ORF3 protein expressed in E. coli strain BL21. (C) Western blot analyses of GST-ORF3 protein and MAbs against ORF3-encoding protein. Lane 1, GST-ORF3 protein recognized with rabbit anti-GST polyclonal antibody; lane 2, positive control reacted with rabbit anti-GST polyclonal antibody; lanes 3–6, MAbs 1H3, 3B1, 3D12, and 3G7 against PCV2-ORF3-encoding protein strongly reacted with GST-ORF3 protein expressed in E. coli.