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. 2012 Jun;31(3):180–187. doi: 10.1089/hyb.2011.0116

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 4. — PK-15 cells transfected with the vectors pEGFP-C2-F3 and pCI-noe-F3. (A) PK-15 cells transfected with pEGFP-C2 vector. (B) GFP-ORF3 fusion protein was expressed in PK-15 cells at 9 h post-transfection. (C) PK-15 cells expressing GFP-ORF3 fusion protein shrank and segmented at 48 h post-transfection. (D–G) PK-15 cells transfected with the recombinant plasmid pCI-neo-F3 was recognized specifically by MAbs 1H3, 3B1, 3D12, and 3G7 in IHC analysis. (H) PK-15 cells transfected with the vector pCI-neo in IHC analysis.