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RT-PCR analysis of ORF3 mRNA transcription in PCV2-infected PK-15 cells. Lanes 1, 3, 5, 7, and 9, RT-PCR products of ORF3 were amplified at 12, 24, 36, 48, and 60 h.p.i. from the total RNA of PCV2-infected PK-15 cells. Lanes 2, 4, 6, 8, and 10, PCR products were amplified at 12, 24, 36, 48, and 60 h.p.i. from the total RNA of PCV2-infected PK-15 cells to avoid any DNA contamination as a negative control; lane 11, RT-PCR product of PK-15 cells; lane 12, PCR product of PK-15 cells.
