Table 1.
Oligonucleotide Primers in This Study
| Primer | Primer sequencea | Application |
|---|---|---|
| F3GZU1 | >5′-GCGAATTCATGGTAACCATCCCACCACTTGTTTCTCGAT-3′ | Amplification of ORF3 DNA sequence |
| F3GZD1 | <5′-TGTGCGGCCAGCGCGGTGTGGTAA-3′ | Rare codon modification of ORF3 DNA sequence |
| F3GZU2 | >5′-TACCACACCGCGCTGGCCGCACAATGA-3′ | Rare codon modification of ORF3 DNA sequence |
| F3GZD2 | <5′-GGCCTCGAGTTACTTATTGAATGTGGAGCTTCGCGATCGCAA-3′ | Amplification of ORF3 DNA sequence |
| F3TF3 | <5′-GCGGATCCTTACTTATTGAATG-3′ | pEGFP construction |
| F671 | >5′-ATGGTAACCATCCCACCACTTG-3′ | RT-PCR |
| R357 | <5′-TTACTGATGGAGTGTGGA-3′ | RT-PCR |
| F-sac2 | >5′-GAACCGCGGGCTGGCTGAACTTTTGAAAGT-3′ | Amplification of full-length PCV2 genome |
| R-658 | <5′-TTCTTCACCTTGGTAACCATC-3′ | Mutation of ORF3 initiation codon |
| F661 | >5′-ATGGTTACCAAGGTGAAGAAGT-3′ | Mutation of ORF3 initiation codon |
| R-sac2 | <5′-GCACCGCGGAAATTTCTGACAAACGTTACA-3′ | Amplification of full-length PCV2 genome |
a
Primer direction is designated at beginning of sequence.