FIG. 1.

SZ117 recognizes a 280 kDa protein in Matrigel and tumor cells. The 280, 250, 150, and 53 kDa bands in tumor cell SW480 and Matrigel were detected by Western blotting using SZ117 (A, left panel) or commercial filamin A monoclonal antibody (A, right panel). The expression of 280 kDa, 150 kDa, and 53 kDa proteins were also observed in various tumor cell lines, including ECV304, U87-MG, HMY2-CIR, Raji, HEL, NCI-H292, 95-D, UC-CUC, HLMVEC, H9, Jurkat, Hey1B, SW480, and MG63 (B). Of note, β-actin was not detected in H9 cells by the antibody.