Table 1.
Identification of the seroreactive proteins of C. burnetii by MALDI-TOF-MS and ESI-MS/MS
| spot no | Identification | Gene name | Locus tag | NCBI no. | Nominal mass | Calculated pI value | Identify method | Score | Expect value | Queries matched | %Sequence coverage | Mice sera (-days-p.i.) | Human sera(A,B) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Chaperone protein | dnaK | CBU_1290 | gi|29654590 | 70826 | 5.14 | MALDI-TOF | 176 | 6.80E-12 | 21 | 38% | - | A,B |
| 2 | Chaperonin GroEL | groEL | CBU_1718 | gi|161830449 | 58375 | 5.14 | MALDI-TOF | 200 | 2.70E-14 | 24 | 52% | 14,21,28 | A,B |
| 3 | Trigger factor | tig | CBU_0737 COXBURSA | gi|29654071 | 50215 | 5.3 | MALDI-TOF | 223 | 1.40E-16 | 32 | 67% | 28 | A,B |
| 4 | F0F1 ATP synthase subunit beta | atpD | 331_A2148 | gi|161830152 | 50490 | 5.01 | MALDI-TOF | 240 | 2.70E-18 | 26 | 54% | 21,28 | A,B |
| 5 | Dihydrolipoyllysine-residue succinyltransferase | sucB | CBU_1398 | gi|29654691 | 45908 | 5.54 | MALDI-TOF | 100 | 0.00027 | 16 | 34% | 21,28 | A |
| 6 | Fructose-1,6-bisphosphate aldolase | fbaA | CBU_1778 | gi|29655066 | 39793 | 5.41 | MALDI-TOF | 190 | 2.70E-13 | 16 | 48% | 21,28 | A,B |
| 7 | S-adenosylmethionine Synthetase | metK | CBU_2030 | gi|29655311 | 43150 | 5.55 | MALDI-TOF | 153 | 1.40E-09 | 20 | 50% | - | A,B |
| 8 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 | fabF | CBU_0497 | gi|29653839 | 44275 | 5.49 | MALDI-TOF | 160 | 2.70E-10 | 20 | 58% | - | A |
| 9 | Elongation factor Tu | tuf2 | CBU_0236 | gi|29653588 | 43613 | 5.32 | MALDI-TOF | 285 | 8.60E-23 | 29 | 76% | 28 | A,B |
| 10 | Glutamine synthetase | glnA | CBU_0503 | gi|29653845 | 39876 | 5.33 | MALDI-TOF | 122 | 1.7e-06 | 15 | 44% | - | A |
| 11 | Malate dehydrogenase | mdh | CBU_1241 | gi|29654544 | 35732 | 5.07 | MALDI-TOF | 136 | 6.80E-08 | 19 | 50% | 21,28 | A |
| 12 | 34 kDa outer membrane protein | ybgF | - | gi|30025849 | 33641 | 5.67 | MALDI-TOF | 92 | 0.0019 | 8 | 28% | 21,28 | A |
| 13 | (2R)-phospho-3-sulfolactate synthase | comA | CBU_1954 | gi|29655237 | 33383 | 5.38 | MALDI-TOF | 146 | 6.80E-09 | 16 | 52% | 28 | A |
| 14 | Inorganic diphosphatase | ppa | CBU_0628 | gi|29653966 | 19642 | 5.2 | ESI-MS/MS | 323 | 2.1e-26 | 7 | 36% | 28 | - |
| 15 | LSU ribosomal protein L12P (L7/L12) | rplL | CBU_0229 COXBURSA | gi|29653581 | 13240 | 4.71 | ESI-MS/MS | 210 | 4.2e-15 | 6 | 48% | - | A,B |
| 16 | 30S ribosomal protein S2 | rpsB | 331_A1545 | gi|161831161 | 35410 | 8.88 | MALDI-TOF | 100 | 0.00027 | 15 | 48% | 28 | - |
| 17 | Peptidyl-prolyl cis-trans isomerase Mip | mip | CBU_0630 | gi|29653968 | 25501 | 9.8 | MALDI-TOF | 133 | 6.10E-07 | 9 | 57% | 14,21,28 | - |
| 18 | 27 kDa outer membrane protein | com1 | - | gi|11935138 | 26739 | 9.23 | MALDI-TOF | 95 | 0.00078 | 7 | 42% | 14,21,28 | - |
| 19 | Acute disease antigen A | adaA | CBU_0952 | gi|29654269 | 25935 | 8.67 | MALDI-TOF | 110 | 2.70E-05 | 15 | 38% | - | B |
| 20 | Putative outer membrane Skp | ompH | CBU_0612 | gi|29653950 | 18812 | 9.71 | ESI-MS/MS | 429 | 4.3e-37 | 5 | 28% | 14,21,28 | - |